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Last updated: 2022-04-07

Checks: 2 0

Knit directory: Fetal-Gene-Program-snRNAseq/

This reproducible R Markdown analysis was created with workflowr (version 1.7.0). The Checks tab describes the reproducibility checks that were applied when the results were created. The Past versions tab lists the development history.

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R Markdown file: up-to-date

Great! Since the R Markdown file has been committed to the Git repository, you know the exact version of the code that produced these results.

Repository version: 78db7d6

Great! You are using Git for version control. Tracking code development and connecting the code version to the results is critical for reproducibility.

The results in this page were generated with repository version 78db7d6. See the Past versions tab to see a history of the changes made to the R Markdown and HTML files.

Note that you need to be careful to ensure that all relevant files for the analysis have been committed to Git prior to generating the results (you can use wflow_publish or wflow_git_commit). workflowr only checks the R Markdown file, but you know if there are other scripts or data files that it depends on. Below is the status of the Git repository when the results were generated:

working directory clean

Note that any generated files, e.g. HTML, png, CSS, etc., are not included in this status report because it is ok for generated content to have uncommitted changes.

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These are the previous versions of the repository in which changes were made to the R Markdown (analysis/about.Rmd) and HTML (docs/about.html) files. If you’ve configured a remote Git repository (see ?wflow_git_remote), click on the hyperlinks in the table below to view the files as they were in that past version.

File	Version	Author	Date	Message
Rmd	78db7d6	neda-mehdiabadi	2022-04-07	wflow_publish(c("analysis/Rmd", "data/txt", "data/README.md",
Rmd	040c27b	neda-mehdiabadi	2022-04-06	Start workflowr project.

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This is a large single nuclei RNA-seq dataset generated by Evangelyn Sim and Enzo Porrello from the Heart Regeneration group at the Murdoch Children’s Research Institute (MCRI). The samples were prepared by Evangelyn Sim and the data was processed and sequenced at the Translational Genomics Unit at MCRI.

This analysis will focus on data generated from the left ventricle from three groups: fetal (n=3), non-diseased (n=3), and DCM (n=4). There are several aspects of the analysis:

• Define the cell types present at each group.
• Analyse differences in cell type composition across these groups.
• Identify transcriptional pathways perturbed in DCM.
• Define fetal gene program in dilated cardiomyopathy.

Also, we have developed an interactive website that permits interrogation of our data set and, hence, increases the data set’s accessibility and utility. To check our website, please visit the Heart Explorer.